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KMID : 0381120160380040333
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Genes and Genomics
2016 Volume.38 No. 4 p.333 ~ p.340
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Overexpressing the wheat dihydroflavonol 4-reductase gene TaDFR increases anthocyanin accumulation in an Arabidopsis dfr mutant
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Shin Dong-Ho
Choi Myoung-Goo
Kang Chon-Sik
Park Chul-Soo
Choi Sang-Bong
Park Youn-Il
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Abstract
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Plants of wheat cultivar Iksan370, which was recently produced by crossing paternal line Keumkang with maternal line Xian83, accumulate anthocyanins in their coleoptiles and seeds. However, the spatio-temporal regulation of anthocyanin biosynthesis genes in wheat remains poorly understood. Therefore, in the present study, we characterized wheat dihydroflavonol 4-reductase (DFR), which catalyzes the conversion of dihydroflavonol to leucoanthocyanidins during anthocyanin biosynthesis, using a heterologous expression system. The deduced amino acid sequence from full-length cDNA of wheat DFR cloned from young seedlings of Iksan370 (TaDFR-I) includes a well-conserved substrate-binding domain compared with previously identified DFRs. Furthermore, as expected, phylogenetic tree analysis placed TaDFR-I in the monocotyledonous clade. Introduction of TaDFR-I into the wild-type Arabidopsis Col-0 and dfr mutant backgrounds resulted in significant accumulation of anthocyanins in the respective transgenic plants. Similarly, TaDFR transcripts highly accumulated in Iksan370 wheat seedlings under various anthocyanin-inducing conditions, including low temperature, high salt and sugar levels, and UV-B illumination. Thus, we functionally characterized TaDFR-I from Iksan370 lines using a heterologous expression system and revealed the presence of transcriptional regulatory factors similar to those of Arabidopsis that regulate TaDFR expression in response to various abiotic stresses.
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KEYWORD
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Anthocyanin biosynthesis, Wheat dihydroflavonol 4-reductase, Transgenic Arabidopsis
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